Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Synergistic Antioxidant and Anti-Inflammatory Effects between Modified Citrus Pectin and Honokiol

doi: 10.1155/2017/8379843

Figure Lengend Snippet: Inhibition of LPS-induced TNF- α (pg/ml) production by HNK, MCP, and MCP : HNK (9 : 1) in RAW 264.7 mouse monocyte cell line. The cells were treated with compounds and/or LPS in starvation medium and TNF- α analyzed by ELISA. Inhibition curves were analyzed by paired t test; ∗ p < 0.05 for HNK versus MCP, and HNK versus MCP : HNK (9 : 1); S, synergism between MCP and HNK.

Article Snippet: TNF- α produced and secreted into the medium by the cells was analyzed by ELISA protocol using the mouse TNF- α Quantikine ELISA kit (R&D systems, Minneapolis, MN) as per manufacturer's instructions [ ].

Techniques: Inhibition, Enzyme-linked Immunosorbent Assay

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Synergistic Antioxidant and Anti-Inflammatory Effects between Modified Citrus Pectin and Honokiol

doi: 10.1155/2017/8379843

Figure Lengend Snippet: Dose-effect relationship between modified citrus pectin (MCP) and honokiol (HNK).

Article Snippet: TNF- α produced and secreted into the medium by the cells was analyzed by ELISA protocol using the mouse TNF- α Quantikine ELISA kit (R&D systems, Minneapolis, MN) as per manufacturer's instructions [ ].

Techniques: Modification, Inhibition, Antioxidant Activity Assay, Activity Assay

Journal: iScience

Article Title: Amitriptyline revitalizes ICB response via dually inhibiting Kyn/Indole and 5-HT pathways of tryptophan metabolism in ovarian cancer

doi: 10.1016/j.isci.2024.111488

Figure Lengend Snippet: Expression of MYD88, IDO1, and IL4-I1 in EOC cells after LPS-induced combination with AMI (A−C) RT-qPCR and Western blot verification of MYD88 and IDO1 expression in A2780, OVCAR3, and ID8 EOC cells after LPS-induction (1 μg/ml). (D−F) mRNA expression of MYD88 and IDO1 by the combination of LPS with AMI in A2780, OVCAR3 (AMI: 50, 100μm) and ID8 (AMI: 30, 60μm) EOC cells. (G−H) MYD88 and IDO1 expression was not detected in A2780 EOC cells according to the pre-WB results. Therefore, only the expression of MYD88 and ID8 protein levels in OVCAR3 and ID8 were performed in the combined treatment. (Protein expression of MyD88 and IDO1 relative to Tubulin in EOC cells treated with different concentrations of drugs). (I) mRNA expression of IL4I1 on A2780, OVCAR3, and ID8 EOC cells after LPS combined with AMI treatment was analyzed by RT-qPCR, in which IL4I1, as a secreted protein, was detected in the levels in the EOC cells culture supernatant with Elisa. Each assay was performed in triplicate, and data are presented as mean ± SD ( n = 3).

Article Snippet: ELISA kit- TNF-α (Mouse) , Novus Biologicals , VAL609.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Amitriptyline revitalizes ICB response via dually inhibiting Kyn/Indole and 5-HT pathways of tryptophan metabolism in ovarian cancer

doi: 10.1016/j.isci.2024.111488

Figure Lengend Snippet: The killing activity of T lymphocytes against EOC cells and expression of CD39 after AMI stimulation (A) Expression of CD8 + CD39 + in T lymphocytes after exposure to different concentrations of AMI-treated (AMI concentrations refer to the preceding) EOC cells culture supernatants for 12 or 24 h as analyzed by FCM. (B) The killing activity of corresponding T lymphocytes (effector cells) to parental EOC cells (target cells) or AMI-treated EOC cells (target cells) shown are from the effector/target ratio at 1:1, 5:1, and 10:1. Supernatant 0h, 24h, and 48h represent the stimulation of T lymphocytes by supernatants collected at different times from EOC cells treated with AMI, respectively. (C) Measure the levels of TNF-α and IFN-γ in lymphocyte culture supernatants stimulated with AMI-treated EOC cell culture supernatants by Elisa assays. Each assay was performed in triplicate, and data are presented as mean ± SD ( n = 3). In Elisa results, ∗ indicates compared to the lymphocytes without any treatment (LN), # indicates compared to the lymphocytes stimulated with DMSO-treated EOC cell culture supernatant. #, ##, ### and #### indicates p < 0.05, p < 0.01, p < 0.001, and p < 0.0001 respectively.

Article Snippet: ELISA kit- TNF-α (Mouse) , Novus Biologicals , VAL609.

Techniques: Activity Assay, Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Amitriptyline revitalizes ICB response via dually inhibiting Kyn/Indole and 5-HT pathways of tryptophan metabolism in ovarian cancer

doi: 10.1016/j.isci.2024.111488

Figure Lengend Snippet: AMI reshapes TIME, the serum level of IL-4I1 and TRP metabolites peripheral blood of EOC-bearing mice (A and B) Tryptophan metabolites in the serum of unimplanted tumor-bearing mice (normal mice) and compared with implanted tumor-bearing mice (controls). (C and D) Tryptophan metabolites in the serum of normal mice (without implanted tumors) before and after the use of AMI. (E−H) EOC-bearing mice receiving PD-1 inhibitor - combined AMI therapy, LC-MS/MS was used to detect the amount of KYN, KYNA, TRP, and the ratio of KYN/TRP in the serum of EOC-bearing mice. (I) IL4I1 in EOC-bearing mice serum by Elisa. (J) LC-MS/MS was used to detect the amount of AHR ligands (I3P, IAA, I3A, and ILA) in the serum of EOC-bearing mice. Data are presented as mean ± SD ( n = 3).

Article Snippet: ELISA kit- TNF-α (Mouse) , Novus Biologicals , VAL609.

Techniques: Liquid Chromatography with Mass Spectroscopy, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Amitriptyline revitalizes ICB response via dually inhibiting Kyn/Indole and 5-HT pathways of tryptophan metabolism in ovarian cancer

doi: 10.1016/j.isci.2024.111488

Figure Lengend Snippet:

Article Snippet: ELISA kit- TNF-α (Mouse) , Novus Biologicals , VAL609.

Techniques: Recombinant, Bicinchoninic Acid Protein Assay, Membrane, Isolation, Enzyme-linked Immunosorbent Assay, Lactate Dehydrogenase Assay, Software

Journal: Frontiers in Immunology

Article Title: Aqueous Artemisia argyi extract mitigates acute lung injury in association with coordinated alterations in gut microbiota, metabolic homeostasis, and pulmonary inflammatory gene expression

doi: 10.3389/fimmu.2026.1770675

Figure Lengend Snippet: AEAA pretreatment is associated with reduced pathological features of LPS-induced acute lung injury in mice. (A) Experimental design of the LPS-induced acute lung injury (ALI) model. (B) Histopathological evaluation of lung tissues. Representative H&E-stained sections from different treatment groups at 20× and 40× magnification show alveolar wall thickening, inflammatory cell infiltration, hemorrhage, and edema in LPS-treated mice. AEAA treatment dose-dependently ameliorated these pathological alterations (red boxed areas). (C) Wet-to-dry (W/D) lung weight ratio. (D) Lung injury score. (E–G) Pro-inflammatory cytokines in serum and BALF.IL-1β (E) , IL-6 (F) , and IL-10 (G) levels in serum and BALF. (H) TNF-α levels in serum and BALF. (I) Myeloperoxidase (MPO) activity. Data are presented as mean ± SEM. n = 5 per group. Statistical analysis was performed using one-way ANOVA followed by post hoc tests. * P < 0.05 , ** P < 0.01 , *** P < 0.001 , **** P < 0.0001 .

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) kits for mouse IL-1β (Cat. #EK201B), IL-6 (Cat. #EK206), IL-10 (Cat. #EK210), TNF-α (Cat. #EK282), and myeloperoxidase (MPO) (Cat. #EK2133) were obtained from Multisciences (Lianke) Biotech Co., Ltd. (Hangzhou, China).

Techniques: Staining, Activity Assay

Journal: Frontiers in Immunology

Article Title: YiQi-HuoXue prescription ameliorates LPS-induced sepsis-associated encephalopathy via VCAM-1–mediated microglial efferocytosis

doi: 10.3389/fimmu.2026.1792688

Figure Lengend Snippet: Pharmacological modulation of MERTK and VCAM1 implicates a VCAM1-linked efferocytosis and anti-inflammatory mechanism for YQHXP. (A) Flow cytometry analysis of PKH67 + /F4/80 + double-positive cells in BV2–HT22 co-culture across seven groups. (B) Quantification of efferocytosis rate(n=3). (C) Flow cytometry plots of apoptotic BV2 cells (Annexin V/PI staining). (D) Quantification of apoptosis rate(n=3). (E) Western blot and quantification of C1QB protein expression(n=3). (F) Western blot and quantification of MERTK protein expression(n=3). (G) Western blot and quantification of VCAM1 protein expression(n=3). (H) TNF-α levels in BV2 supernatant measured by ELISA(n=3). IL-6 levels in BV2 supernatant measured by ELISA(n=3). *P < 0.05 relative to the model group; **P < 0.01, ***P < 0.001 relative to the model group. # P < 0.05 relative to the UNC2250 group; ## P < 0.01 relative to the UNC2250 group; ### P < 0.001 relative to the UNC2250 group.

Article Snippet: Mouse TNF-α (Cat. E-EL-M3063) and IL-6 (Cat. E-EL-M0044) ELISA kits were sourced from Elabscience Biotechnology Co., Ltd. (Wuhan, China).

Techniques: Flow Cytometry, Co-Culture Assay, Staining, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay